Compose a 1250 words assignment on determination of calcium. Needs to be plagiarism free! The expressions below are quoted directly from the Biophysical Journal. Pluronic-127 for 30–60 min at room temperature, followed by a 30-min recovery in EBSS at 37°C. Images were acquired using a high-speed Photometrics CCD camera (frame transfer EEV37 chip) on an inverted Zeiss microscope, controlled by Innovision software on a Silicon Graphics workstation. Coverslips were mounted on an open-welled chamber. The cells were bathed in pH-controlled EBSS and maintained at 37°C. Ratio pairs of 340- and 380-nm excitation were collected for some experiments, with emission passed through a rhodamine filter. Using this acquisition protocol, ratio pairs could be collected approximately once a second. Because the BK-induced calcium waves are typically very fast, faster imaging was necessary to resolve the geometry of the calcium wave propagation. Faster imaging was possible using only the 380-nm excitation wavelength, in which images could be obtained every 65 ms. Cell exposure
during excitation was kept to a minimum by using 4 3 4 binning (resulting in 128 3 128 images) and increased gain settings, but always maintaining at least a 5-to-1 cell-to-background intensity ratio. BK was added in 20-ml aliquots to the cell chamber so that the exposure was nearly instantaneous but with as little cellular disturbance as possible.” (Charles et al, 2000).
Physical properties: Calcium is a silvery solid, metal and has a melting point of about 64 and a boiling point of 773. It can act as a reducing agent. And it reacts slowly with water. Calcium is a divalent element with each of its atom having two valence electrons.
1. Flame Test: This is a procedure used in Chemistry to detect if some impurities are present in a particular substance. The test itself is simple and requires a few materials. Some of the materials needed are a chrome or Platinum wire, concentrated hydrochloric acid and a Bunsen burner that produces a flame.